ABSTRACT
BACKGROUND: Distraction osteogenesis is an effective method for large bone defects, bone tumors and osteomyelitis. However, there is a lack of a standard model in the basic research concerning distraction osteogenesis. OBJECTIVE: To establish a rabbit femoral model of distraction osteogenesis and to assess its osteogenic effect. METHODS: Twenty male New Zealand rabbits were enrolled to establish the rabbit femoral model of distraction osteogenesis using a novel distractor. Subsequently, the gross observation and X-ray examination of the specimens were performed to assess the osteogenic effect. RESULTS AND CONCLUSION: Gross observation: on day 14 after fixation, there were light-colored and dense newborn bones distributing evenly in the distraction gap, and appeared with a columnar connection with the broken ends; the boundary with normal bones became vague. On day 35, the surface of newborn bones in the distraction gap showed the same color and texture with the normal ones, the boundary between the newborn and normal bones was difficult to distinguish, and the bone mineral density was increased notably. Radiology results: on day 14 after fixation, the stent was fixed stably, the broken ends got good reduction, and cloudy shallows connecting the two ends of the normal bones in the distraction gap and increased bone mineral density were detectable. Completely calcified new-born bones, intact bone cortex and open medullary canal were further visible on day 35. These results suggest that the rabbit femoral model of distraction osteogenesis is established successfully using the self-designed single-arm distractor based on rational surgical procedures and standard operations.
ABSTRACT
Pterocypsela is a very important traditional Chinese medicine from the tribe Cichorieae of Asteraceae. Mitotic chromosome numbers and karyotypes are reported for P. formosana and P. elata from Hunan and Hubei province, China. The former is new and the latter provide confirmation of previous reference. All P. taxa are diploidy with 2n = 18 and their basic number is tentatively suggested as x = 9. Karyotype of Pterocypsela is 2A and P. formosana with a karyotype formula of 2n = 2x = 18 = 4m + 14sm, and 2n = 2x = 18 = 2m + 8sm +8st for P. elata. It is the first time to report the AI value for Pterocypsela in this paper. Cytological data of chromosomal numbers and karyotypes were used to discuss the close relationships of the Pterocypsela genus and the taxonomy of the medicinal plants.
Subject(s)
Asteraceae , Classification , Cell Biology , Genetics , Chromosomes, Plant , Genetics , Diploidy , KaryotypingABSTRACT
Objective To approach the effect of benefiting qi and activating blood circulation therapy on AMI.Methods A total of 120 patients with AMI were recruited into a control group and a treatment group randomly. 60 cases in control group were treated with dextran, nitroglycerin and heparin. While 60 cases in the treatment group were treated with traditional Chinese medicines with the functions of benefiting qi and activating blood circulation on the basis of the control group. Therapeutic effects were observed after two weeks' treatment. Results The effective rat was 95.00% and 81.67% in the treatment group and the control group respectively, showing significant difference(P=0.0341 ＜0.05). In the 3 months follow-up, ischemia happened in the treatment group was significantly lower than the control group (P=0.0284, P＜0.05).Conclusion Combined treatment of TCM and western medicine is better than treatment of western medicine only. Benefiting qi and activating blood circulation therapy is effective on AMI and improves the prognosis.
ABSTRACT
CD72 is a B cell specific receptor that exists in multiple alternative splicing forms. Eight novel alternative splicing forms of CD72 were identified from the spleenocytes of BALB/C mice. Two very unique intron sequences were found in those alternative splicing forms. One kind of splicing variants retained the intron1 in the mRNA. This intron can be translated into 32 amino acid residues without changing the reading frame of the whole proteins. Another kind of splicing variants used an alternative 3' splice site in intron 3(3'AS) which led to premature termination of its encoded protein. The differential expression of the CD72 splicing variants were compared in BALB/C and NZB/W mice that were at different stage of systematic lupus erythematosis(SLE) disease development. It was found that 1) splicing forms containing 3'AS was rare in all samples examinated; 2) splicing forms containing two ITIM domains and transmembrane domains were more abundant in BALB/C mice than in NZB/W mice, even in some cases the two ITIM domains were separated by the intron 1; 3) a shorter splicing form with both exon2 and exon3 missing was expressed highly in terminally diseased NZB/W mice.These results suggested an important role of CD72 alternative splicing forms in B cell receptor signaling and in SLE.
ABSTRACT
Objective To investigate the relationship between Demodex infection and season , sex, different parts and the infectious rate of Demodex in human faces among students of a university and to prevent diseases caused by Demodex infection.Methods The samples of Demodex were collected from sophomores in December 2006 to May 2007.Results The results showed that the infectious rate in male,31.5% in winter and 38.0% in summer respectively, higher than those in female.On the face, the highest infectious rate was on the nose.The infectious rate on the cheeks was lowest.The relationship between Demodex infection and season, sex was observed.Conclusion The infectious rate of Demodex in human faces is associated with the season and sex.